productdatasheet
CatalogNo.:2426
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
NuclearfactorkappaB(NF-κB)isaubiquitoustranscriptionfactorandkeymediatorofgeneexpressionduringimmuneandinflammatoryresponses.NF-κBactivatesnumerousgenesinresponsetoextracellularstimuli,suchasIL-1,TNFα,LPS,oxidativestress,andmitogens.NF-κBisassociatedwithIκBincytoplasm.Afterstimulation,1κBisphosphorylatedanddissociatesfromNF-κB.NF-κBenterscellnucleiwhereitactivatesanarrayofgenes.Aserine/threonineproteinkinaseassociatedwithIL-1receptor(IRAK)mediatesasignalingcascadeleADIngto
NF-κBactivation.IRAKisassociatedwithIL-1receptorsubunitsIL-1RIandIL-1RacPandservesasasignalingmoleculetomediateIL-1responses.IRAKalsomediatesIL-18-inducedNF-κBactivation.
SPECIFICATIONSUMMARYAntigen:PeptidecorrespondingtheC-terminusofhumanIRAK.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhumanIRAK(80kD).
Immunoblotting:useat1:1,000-1:2,000dilution.
Immunoprecipitation:use2-4ugantibodypersample.
Positivecontrol:WholecelllysatefromHeLacellsorTHP-1cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
QEDBioscience,Inc.10919TechnologyPlace,SuiteC
SanDiego,CA92127
TollFree800.929.2114
Phone858.675.2405
Fax858.592.1509
info@qedbio.com
Visitourwebsiteforadditionalproductinformationandtoorderonline.
qedbio.com
