
IRAK-4PolyclonalAntibody
CatalogNo.:56412
Size:100ugIgG(1mg/ml)inPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
IRAK-4activatestheNF-BandMAPKpathwaysandplaysaroleinIL-1Rmediatedinflammatoryresponsesandinnateimmunity.IRAK-4-deficientanimalsareresistanttochallengewithLPSandareimpairedintheirresponsestoviralandbacterialchallenges.
Antigen:AsyntheticpeptidecorrespondingtoaminoacidsatthecarboxyterminusofhumanIRAK-4.HostSpecies:RabbitPreservative:0.02%sodiumazide
ThisantibodyspecificallyrecognizeshumanIRAK-4(IL-1ReceptorAssociatedKinase-4).Thisantibodydoesnotcross-reactwithotherIRAKs.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem
Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.
APPLICATIONSImmunoblotting:Useat1-4ug/ml.Abandofapproximately50kDaisdetected.
IRAK-4inHeLacelllysatedetectedwith#56412at1,2,and3ug/ml.
Immunocytochemistry:Useat10ug/ml.
IRAK-4inK562cellswith#56412at10ug/ml.
Immunofluorescence:Useat10ug/ml.
IRAK-4inK562cellswith#56412at10ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Stableforoneyearat-20C.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.