productdatasheet
CatalogNo.:2213
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
Thepro-inflammatorycytokineIL-1inducescellularresponsesthroughtwosubunitsofitsreceptor,IL-1receptor1(IL-1R1)andIL-1receptoraccessoryprotein(IL-1RacP).IL-1receptor-associatedkinase(IRAK)mediatesactivationofNF-κB,akeytranscriptionfactorthatmediatesinflammatoryandimmunerespones.AnovelmemberoftheIRAK/PellefamilywasrecentlyidentifiedanddesignatedIRAK2.BothIRAKandIRAK2associatewiththesubunitsoftheIL-1RcomplexafterIL-1bindingandleadtoNF-κBactivation.
IRAKsalsoassociatewithToll-like
receptor(TLR)andthedominantnegativemutantsofIRAKsinhibitLPS-inducedNF-κBactivation.MembersoftheIRAK/PellefamilyplayacentralroleinIL-1RandTLR-mediatedinflammatoryresponsestoIL-1andLPS.IRAK2isexpressedinavarietyofhumantissues.
SPECIFICATIONSUMMARYAntigen:Peptidecorrespondingtoaa571-590ofhumanIRAK2.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanIRAK2(65kD).Nocross-reactivitywithIRAK.
Immunoblotting:useat1:500-1:1,000dilution.
Positivecontrol:WholecelllysatefromHeLacellsorK562cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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