Catalogno.:11078

Size:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4.,0.09%sodiumazide.

Antigen:Peptidecorrespondingtoaa192-212ofbovineKDELreceptorconjugatedtoKLH.

HostSpecies:Mouse

Subclass:IgG1

CloneNumber:KR-10

KDELreceptor,anintegralmembraneprotein,facilitatestheretrievalofsolubleendoplasmicreticulum(ER)luminalproteinsbearingthefouraminoacidretentionmotifKDELfromtheGolgiapparatus.ThesequenceofKDELreceptorisverysimilartothatoftheyeastproteinErd2p,areceptorforproteinscontainingthetetrapeptideHDEL,theyeastERretentionsignal.

KDELreceptorsinteractwithGAP(GTPase-activatingprotein)ofARF1,asmallGTP-bindingproteininvolvedinCOPI-dependentvesicletransport.

KDELreceptorsmayrecruitcytosolicARF1tomembranesandregulatevesiclebudding.

Thisantibodyrecognizeshuman,monkey,mouse,rat,hamster,rabbit,porcine,bovine,ovine,canine,chicken,Drosophila,andXenopusKDELreceptor1.

Westernblot:Useat1:1,000dilution.Abandofapprox.25kDaisdetected.

Immunocytochemistry:Useat1:1,000dilution.

Immunoprecipitation:Useat1:500dilution.

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

Storefrozenproductatorbelow-20oC.Thawedproductmaybestoredfor2-4weeksat4oC.Foroptimalstorage,aliquottosmallerportionsandstoreat

-20oCto-70oC.Avoidrepeated

freeze/thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.