productdatasheet
CatalogNo.:28108
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
Kinaseofembryonicstemcells(Kos1)isanon-receptorproteintyrosinekinase(NRPTK)andanewmemberoftheAckfamilyofNRPTKs.Kos1isaubiquitouslyexpressed47-kDaproteinwithautotyrosinekinaseactivitythatisdevelopmentallyregulatedduringembryogenesis.Kos1isalsoupregulatedfollowingIL3withdrawalfromfactor-dependentmurineNSF/N1.H7cellsthatundergoapoptosis,suggestingaroleingrowthinhibition.DatasupportanegativeregulatoryroleforKos1inregulatingtheRas-Raf1-MAPKgrowthpathwaybyamechanismthatrequiresitsautotyrosinekinaseactivity.
Immunogen:Syntheticpeptidecorrespondingtoaminoacids421-434(QNQVQSLPAGSAEP)ofmouseKos1conjugatedtoKLHattheN-terminus.HostSpecies:Rabbit
StABIlizers:NonePreservatives:None.Availableonrequest.
ThisantibodyrecognizesmouseKos1protein.
Immunoblotting:useat1:500dilution.Abandofapproximately47kDaisdetected.
Positivecontrol:transfectedcelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Hoare,Ketal.(2003)Oncogene22:3562-3577.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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