Anti-LeadMonoclonalAntibody
CatalogNo.:60001(clonePb1).
Size:0.5mlascites.
Exposuretopotentiallyneurotoxiclevelsofleadoccursinapproximately9%ofAmericanchildrenunder6yearsofage,andmanyurbandwellingsstillcontaindangerouslevelsofleadpaint.
Diagnosisandtreatmentofchronicleadexposurehasbeenhamperedbythelackofaclearunderstandingofthespecificmechanismofactionofthistoxicmetal.QEDBioscience’smonoclonalantibodytoleadisthefirstofitskindtobeofferedtothehealthcareandresearchcommunitiesfordevelopmentofimproveddiagnosticassaysofleadtoxicityandforinvestigationsintotheunderlyingmechanismofleadtoxicity.
Antigen:Leadconjugatedtokeyholelimpethemocyanin(KLH)byproprietarychemistry.
HostSpecies:Mouse
AntibodyClass:IgMPreservatives:None.Availableonrequest.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICITYThisantibodydetectsleadintheppbrange.IndirectELISAendpointtiteris
>1:100,000.
IndirectELISA:optimizedforlead-RSA(rabbitserumalbumin)onsolidphaseat200ng/well.Ascitesdiluted1:100,000producesanO.D.signalof0.5.
CompetitionELISA:withlead-RSAonthesolidphaseat200ng/well,50%inhibitionofantibodybindingisachievedwithPb2+at800ng/well.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
Notallapplicationshavebeeninvestigated.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
LinTJandChungMF2008Sensors8:582-593.Referstoproductno.
K55061MwhichisQEDproductno.60001.
