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QED Bioscience/Leucine-Rich Glioma Inactivated Gene 1 (LGI1)/56577/1 Ea
ORDERINGINFORMATION
Catalogno.:56577(cloneS283-7)
Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.
BACKGROUND
Theleucine-richgliomainactivated-1(LGI1)geneisrearrangedasaresultoftranslocationsinglioblastomacelllines.Thisproteincontainsahydrophobicsegmentrepresentingaputativetransmembranedomainwiththeaminoterminuslocatedoutsidethecell.Italsocontainsleucine-richrepeatswithconservedcysteine-richflankingsequences.Thisgeneispredominantlyexpressedinneuraltissuesanditsexpressionisreducedinlowgradebraintumorsandsignificantlyreducedorabsentinmalignantgliomas.
SPECIFICATIONSUMMARY
Antigen:Fusionproteincorrespondingtoaa37-113ofmouseLGI1.Thissequenceis100%identicalinrat,98%identicalinhuman,and~50%identicalinLGI2,LGI3,andLGI4.
Accessionnos:NP_064674.1,Q9JIA1.1
GeneID:56839
HostSpecies:Mouse
AntibodyClass:IgG2a
Specificity:Thisantibodyrecognizeshuman,mouse,andratLGI1.
APPLICATIONS
Immunoblotting:useat1-5ug/ml.Abandof
~60kDaisdetected.
Immunofluorescence:useat10ug/ml.
DetectionofLGI1inratbrainlysatewith#56577at1ug/ml.
DetectionofLGI1inneuroblastomacelllineSK-N-BEwith#56577at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)LGI1stain.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisproductisstableforatleastone(1)yearat-20oC.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
