ORDERINGINFORMATION

Catalogno.:56582(cloneS231B-34)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

Leucine-richrepeatkinase2(LRRK2),alsoknownasdardarin,isanenzymethatinhumansisencodedbythePARK8gene.Theproteinispresentlargelyinthecytoplasmbutalsoassociateswiththemitochondrialoutermembrane.MutationsinthisgenehavebeenassociatedwithParkinson'sdiseasetype8andCrohn’sdiseasewhichsuggeststhatthesetwodiseasesshareacommonpathway.ExpressionofmutantLRRK2inducedapoptoticcelldeathinneuroblastomacellsandinmousecorticalneurons.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa841-960ofhumanLRRK2.Thissequenceis81%identicalinmouse,80%identicalinrat,and<30%identicalinLRRK1.

Accessionnos:NP_940980,Q5S007

GeneID:120892

HostSpecies:Mouse

AntibodyClass:IgG2a

Specificity:ThisantibodyrecognizeshumanandratLRRK2.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

>200kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofLRRK2inratbrainlysatewith

#56582at5ug/ml.

DetectionofLRRK2inneuroblastomacelllineSK-N-BEwith#56582at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)LRRK2stain.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.