CatalogNo.:56499

Size:400ulaffinity-purifiedrabbitimmunoglobulin(250ug/ml)inphosphate-bufferedsaline,pH7.0and50%glycerol.

Post-translationalmodificationsofproteinsplaycriticalrolesintheregulationandfunctionofmanyBIOLOGicalprocesses.Acommonpost-transcriptionalmodificationoflysineismethylation.Lysinecanbemethylatedonce,twice,orthreetimesbylysinemethyltransferases.ThetransferofmethylgroupsfromS-adenosylmethioninetohistonesiscatalyzedbyenzymesknownashistonemethyltransferases.Histonesthataremethylatedoncertainresiduescanrepressoractivategeneexpression.

Antigen:Methylatedlysine-KLHconjugate.

HostSpecies:RabbitAntibodyClass:PolyclonalPreservatives:None.

Thisantibodyrecognizesproteinscontainingmethylatedlysineresidues.Itdoesnotrecognizenon-methylatedproteins.

Immunoblotting:useat0.5-1.0ug/ml

WesternblotanalysisofMethylatedLysineinBSA(left)andMethylatedBSA(right).

ELISA:useat1-10ug/mlwithmethylatedlysine-containingproteinsonthesolidphase.

ImmunoprecipitationImmunohistochemistry

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.