ORDERINGINFORMATION

Catalogno.:56581(clone343-26)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

TheNRCAMgeneencodesaneuronalcelladhesionmoleculewithmultipleimmunoglobulin-likeC2-typedomainsandfibronectintype-IIIdomains.Thisankyrin-bindingproteinisinvolvedinneuron-neuronadhesionandpromotesdirectionalsignalingduringaxonalconegrowth.Thisgeneisalsoexpressedinnon-neuraltissuesandmayplayageneralroleincell-cellcommunicationviasignalingfromitsintracellulardomaintotheactinCytoskeletonduringdirectionalcellmigration.AllelicvariantsofthisgenehavebeenassociatedwithautismandaddictionvulnerABIlity.Alternativesplicingresultsinmultipletranscriptvariantsencodingdifferentisoforms.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa30-845(extracellulardomain)ofmouseNRCAM.Thissequenceis96%identicalinrat,91%identicalinhuman,and~50%identicalinNeurofascin.Accessionnos:NP_001139503.1,Q810U4

GeneID:319504

HostSpecies:Mouse

AntibodyClass:IgG2a

Specificity:Thisantibodyrecognizeshuman,mouse,andratNRCAM.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~160kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofNRCAMinratbrainlysatewith

#56581at1ug/ml.

DetectionofNRCAMinneuroblastomacelllineSK-N-BEwith#56581at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)NRCAMstain.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.