Anti-NIK(CT)Antibody
NuclearfactorkappaB(NF-κB)isaubiquitoustranscriptionfactorandanessentialmediatorofgeneexpressionduringactivationofimmuneandinflammatoryresponses.NF-κBmediatestheexpressionofagreatvarietyofgenesinresponsetoextracellularstimuliincludingIL-1,TNFα,LPS,andmitogens.Aserine/threonineproteinkinasewhichmediatesNF-κBactivationbyIL-1,TNFα,andCD95hasbeenidentifiedanddesignatedNIK(forNF-κBinducingkinase).NIKisanactivatorofIκBkinasealphaandbeta(IKKαandIKKβ).Therefore,NIKisakeymoleculeintheNF-κBsignalingpathwayleADIngtotheexpressionofa
varietyofgeneproductsinresponseto
SPECIFICATIONSUMMARYAntigen:Syntheticpeptidecorrespondingtoaa931-947ofhumanNIK(accessionno.Q99558).HostSpecies:RabbitThisantibodyrecognizeshumanNIK.
Immunoblotting
Recommendedconcentration:2-4ug/ml.Optimaldilutionshouldbedeterminedbyenduser.
Positivecontrol:WholecelllysateofNIK-transfected293cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstablefor3monthsat
oo
pro-inflammatorycytokinesandbacterialproducts.
4Candatleast1yearat-20C.Avoid
multiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
