ORDERINGINFORMATION
CatalogNo.:56456(clone39B6)Format:100uginPBSpH7.4,50%glycerol,and0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.BACKGROUND
NitrotyrosineisaMarkerforinflammationandnitricoxide(NO)productionandisformedinthepresenceoftheactivemetaboliteNO.Mosttyrosinenitrationeventsinvolvecatalysisbymetalloproteinssuchasmyeloperoxidase,eosinophilperoxidase,myoglobin,thecytochromeP-450s,superoxidedismutase,andprostacyclinsynthase.Becausenitrotyrosineisastableproductofmultiplepathways,itsplasmaconcentrationmaybeausefuldeterminantofNO-dependentdamageinvivo.Nitrotyrosinehasbeendetectedininflammatorydiseasessuchassepticshock,rheumatoidarthritis,celiacdisease,atherosclerosis,andchronicrenalfailure.
SPECIFICATIONSUMMARY
Antigen:3-(4-hydroxy-3-nitrophenyl-acetamido)propionicacidconjugatedtoBSA.Accessionno.:NotapplicableHostSpecies:MouseAntibodyClass:IgG2aSpecificity:Thisantibodyrecognizes3-nitrotyrosinemoieties,species-independent.Nodetectablereactivitywithnon-nitratedtyrosine.APPLICATIONS
Immunoblotting:useat1.0ug/ml.Positivecontrol:Linsidomine(SIN-1)-treatedBSA.
Immunohistochemistry:useat1-5ug/ml.
Detectionofnitrotyrosineinratlivertissue.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
