MembersoftheNotchtrans-membraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.TheNotchsignalingnetworkisanintercellularsignalingpathwaythatregulatesinteractionsbetweenphysicallyadjacentcells.TheNotcstrongproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Itfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.ThereisevidencethatactivatedNotch1andNotch3promotedifferentiationofProgenitorcellsintoastroglia.Notch1,activatedbeforebirth,inducesrADIalgliadifferentiation,butpostnatallyinducesthedifferentiationintoastrocytes.

ThisantibodyrecognizesmouseandratNotcstrong.Itdoesnotcross-reactwithNotcstrongorNotcstrong.

Immunoblotting:useat1ug/ml.Bandsof

>270kDa,~120kDa,andsmallerfragments(duetoproteolysis)aredetected.

Positivecontrol:Ratbrainlysate.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.