productdatasheet
CatalogNo.:56472(cloneS59-20)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
BACKGROUNDGlutamatereceptorsoftheNMDAsubtypeareinvolvedinseveralphysiologicalandpathologicalprocessesinthebrain.
FunctionalNMDAreceptorsareheteromultimericcomplexesoftheNR1subunitandoneormoreofthefourNR2subunits(NR2A-D).Coexpressionofbothsubunittypesarerequiredforthefor-mationoffullyfunctionalchannelsthatareinsertedintotheplasmamembrane.TheNR2subunitdeterminescharacteristicsofthechannelsuchasconductance,mean
opentime,andsensitivitytoMg+2.The
ABIlityofNR1andNR2subunitcombinationstofluxCa+2isessentialtotherolethatNMDAreceptorsplayinsynapticplasticityandneurotoxicity.
SPECIFICATIONSUMMARYAntigen:Fusionproteinaa20-271(extracellularN-terminus)ofratNR2B(accessionno.Q00960).Thissequenceis99%identicaltohumanandmouse.HostSpecies:MouseAntibodyClass:IgG2aThisantibodyrecognizeshuman,mouse,andratNR2B.Itdoesnotcross-reactwithNR2A.
APPLICATIONSImmunoblotting:useat1-10ug/ml.Abandof~166kDaisdetected.
LysatesofratbrainandCOS-7cellstransfectedwithempty,NR2A,andNR2Bplasmids.
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/ml
Culturedrathippocampalneuronimmunofluorescence.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Ratbrainlysate
DILUTIONINSTRUCTIONSDiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITYThisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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