productdatasheet
TheMAPK(mitogen-activatedproteinkinase)familyincludesstress-activatedproteinkinases(SAPKs),extracellularsignal-regulatedkinases(ERKs),andp38kinases.Uponactivation,p38translocatestothecellnucleuswhereitphosphorylatesoneormorenuclearsubstratesresultingintranscriptionalchangesandothercellularprocessesinvolvedincellgrowth,division,
differentiation,inflammation,anddeath.p38alwaysactsasapro-apoptoticfactorwithitsactivationleADIngtothereleaseofcytochromecfrommitochondriaandcleavageofcaspase-3anditsdownstreameffectorPARP.p38isactivatedbychemicalstressinducers(suchashydrogenperoxideandheavymetals)andBIOLOGicalstresssignals(suchastumornecrosisfactor,interleukin-1,irradiation,andchemotherapeuticdrugs).
Thisantibodyrecognizeshuman,mouse,rat,bovine,chicken,dog,guineapig,hamster,pig,monkey,rabbit,andsheepp38.
Immunoblotting:useat1:5,000dilution.Abandof~43kDaisdetected.
Immunoprecipitation:useat1:250dilution.Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures
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