ORDERINGINFORMATION

Catalogno.:56576(cloneS233-8)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

PeroxisomalBiogenesisFactor6(PEX6)isamemberoftheAAA(ATPasesassociatedwithdiversecellularactivities)familyofATPases.ItispredominantlyacytoplasmicproteinthatplaysadirectroleinperoxisomalproteinimportandisrequiredforPTS1(peroxisomaltargetingsignal1,aC-terminaltripeptideofthesequenceser-lys-leu)receptoractivity.MutationsinthePEX6genecauseperoxisomebiogenesisdisordersofcomplementationgroup4andcomplementationgroup

6.Severaltranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa2-294(N-terminus)ofhumanPEX6.Thissequenceis82%identicalinratandmouseand<50%identicalwithotherPEXproteins.Accessionnos:NP_000278.3,Q13608

GeneID:5190

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:ThisantibodyrecognizeshumanandratPEX6.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~100kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofPEX6inratbrainlysatewith#56576at5ug/ml.

DetectionofPEX6inneuroblastomacelllineSK-N-BEwith#56576at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)PEX6stain.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.