Anti-PhosphotyrosineMonoclonalAntibody
CatalogNo.:56494(cloneG104))Size:100uginPBS,pH7.4(1mg/ml).PurifiedbyProteinGaffinitychromatography.
Proteinphosphorylationisanimportantposttranslationalmodificationthatservesmanykeyfunctionsinregulationofproteinactivity,localization,andprotein-proteininteractions.PhosphorylationiscatalyzedbyspecificproteinkinasesthatremoveaphosphategroupfromATPandattachitcovalentlytoarecipientprotein.Somekinasesactonserineandthreonine,othersactontyrosine,andmanyactonallthree.Phosphorylationoftyrosineisconsideredoneofthekeystepsinsignaltransductionandregulationofenzymaticactivity.Antibodiestophosphotyrosineareusefulinidentifyingproteinsubstratesoftyrosinekinase.
Antigen:Phosphotyrosine,alanineandglycineina1:1:1ratiopolymerizedinthepresenceofKLHwith1-ethyl-3-(3’-dimethylaminopropyl)carbodiimide.
HostSpecies:Mouse
AntibodyClass:IgG1
SPECIFICITYThisantibodyreactswithphosphotyrosineanddetectsphosphotyrosineinproteinsofunstimulatedandstimulatedcelllysates.
Itdoesnotcross-reactwithphosphoserineorphosphothreonine.
Immunoblotting:useat1ug/ml.Immunohistochemistry:useat1-10ug/ml.Immunofluorescence:useat1-10ug/ml.Positivecontrol:Rattissuelysate
Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
Seespecificproductreferencesbelowforprotocolsandmoreinformation.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
SPECIFICPRODUCTREFERENCES
GartonAJetal.1996MolCellBiol16:6408-6418.
GartonAJandTonksNK1999JBiolChem274:3811-3818.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
