ADVANCEDRESEARCHTECHNOLOGIES

CatalogNo.:56484(clone6H9-B6)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

Piccoloisanovelcomponentofthepresynapticcytoskeletalmatrix(PCM)assembledattheactivezoneofneurotransmitterrelease.PiccoloisamultidomainzincfingerproteinstructurallyrelatedtoBassoon,anotherPCMprotein.BothproteinssharecomponentsofglutamatergicandGABAergicCNSsynapses.Piccolozincfingersinteractwiththedualprenylatedrab3AandVAMP2/SynaptobrevinIIreceptorPRA1.Piccoloisinvolvedinendo-andexocytosisofsynapticvesiclesandhasbeenshowntointerferewithclathrin-mediatedendocytosisbybindingtotheF-actinanddynaminbindproteinAbp1.

Antigen:Full-lengthhumanPiccoloprotein(accessionno.BC001304.1)HostSpecies:Mouse

AntibodyClass:IgG2b

ThisantibodyrecognizeshumanandratPiccoloisoforms(otherspeciesnotyettested).Itdoesnotcross-reactwithBassoon.

Immunoblotting:useat1-10ug/ml.Abandof~550kDaisdetected.

ELISA:useat1-10ug/mlwithPiccoloonthesolidphase.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Ratbrainlysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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