CatalogNo.:56496

Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

Piccoloisanovelcomponentofthepresynapticcytoskeletalmatrix(PCM)assembledattheactivezoneofneurotransmitterrelease.PiccoloisamultidomainzincfingerproteinstructurallyrelatedtoBassoon,anotherPCMprotein.BothproteinssharecomponentsofglutamatergicandGABAergicCNSsynapses.Piccolozincfingersinteractwiththedualprenylatedrab3AandVAMP2/SynaptobrevinIIreceptorPRA1.Piccoloisinvolvedinendo-andexocytosisofsynapticvesiclesandhasbeenshowntointerferewithclathrin-mediatedendocytosisbybindingtotheF-actinanddynaminbindingproteinAbp1.

Antigen:Full-lengthhumanprotein(accessionno.BC001304.1)

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Thisantibodyrecognizeshuman,mouse,andratPiccoloisoforms(otherspeciesnottested).Itdoesnotcross-reactwithBassoon.

Immunoblotting:useat10ug/ml.Abandof~550kDaisdetected.

ELISA:useat1-10ug/mlwithPiccoloonthesolidphase.

Positivecontrol:RatbraintissuelysateThesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.