ORDERINGINFORMATION
Catalogno.:56579(cloneS165-38)
Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.
BACKGROUND
PTPRF,likeothermembersoftheproteintyrosinephosphatase(PTP)family,isasignalingmoleculethatregulatesavarietyofcellularprocesses.PTPRFpossessesanextracellularregion,asingletransmembraneregion,andtwotandemintracytoplasmiccatalyticdomains,andrepresentsareceptor-typePTP.TheextracellularregioncontainsthreeIg-likedomainsandninenon-Iglikedomainssimilartothatofneural-celladhesionmolecule.PTPRFfunctionsintheregulationofepithelialcell-cellcontactsatadherentsjunctionsaswellasinthecontrolofbeta-cateninsignaling.AnincreasedexpressionlevelofPTPRFhasbeenfoundintheinsulin-responsivetissueofobese,insulin-resistantindividualsandmaycontributetothepathogenesisofinsulinresistance.Twoalternativelysplicedtranscriptvariantsofthisgene,whichencodedistinctproteins,havebeenreported.
SPECIFICATIONSUMMARY
Antigen:Fusionproteincorrespondingtoaa1315-1607(cytoplasmicC-terminus)ofhumanPTPRF.Thissequenceis97%identicalinratandmouse,80%identicalinPTPRDandPTPRS,and>50%identicalinPTPRMandPTPRK.
Accessionnos:NP_002831.2,P10586
GeneID:5792
HostSpecies:Mouse
AntibodyClass:IgG2a
Specificity:Thisantibodyrecognizeshuman,mouse,andratPTPRF.
APPLICATIONS
Immunoblotting:useat1-5ug/ml.Abandof
~85kDaisdetectedwhichrepresentsthesubunitcontainingtransmembraneandintracellulardomains.
Immunofluorescence:useat10ug/ml.
DetectionofPTPRFinratbrainlysatewith
#56579at4ug/ml.
DetectionofPTPRFinneuroblastomacelllineSK-N-BEwith#56579at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)PTPRFstain.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisproductisstableforatleastone(1)yearat-20oC.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
