ORDERINGINFORMATION

Catalogno.:56580(cloneS174B-27)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

ThegenethatencodesPCDHGC3isamemberoftheprotocadheringammagenecluster,oneofthreerelatedclusterstandemlylinkedonchromosomefive.Thesegeneclustershaveanimmunoglobulin-likeorganization,suggestingthatanovelmechanismmaybeinvolvedintheirregulationandexpression.Thetandemarrayof22large,variableregionexonsarefollowedbyaconstantregion,containing3exonssharedbyallgenesinthecluster.Eachvariableregionexonencodestheextracellularregion,whichincludes6cadherinectodomainsandatransmembraneregion.Theconstantregionexonsencodethecommoncytoplasmicregion.Theseneuralcadherin-likecelladhesionproteinsmostlikelyplayacriticalroleintheestablishmentandfunctionofspecificcell-cellconnectionsinthebrain.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa720-804(variablecytoplasmicdomain)ofmousePCDHGC3.Thissequenceis97%identicalinhumanand97%identicalinrat.Nosignificantidentitywithotherprotocadherin-gammaproteins.

Accessionnos:NP_291059.1,Q91XX1

GeneID:93706

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:ThisantibodyrecognizeshumanandmousePCDHGC3.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~100kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofPCDHGC3inCOScellstransfectedwithGFP-taggedPCDHGC3with#56580at5ug/ml.

DetectionofPCDHGC3inneuroblastomacelllineSK-N-BEwith#56580at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)PCDHGC3stain.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.