ORDERINGINFORMATION
CatalogNo. | CloneNo. | MAbSubtypeSize |
56457 | 7E3-1B8 | IgG1100ug |
Format:ProteinG-purifiedantibodyinPBS,pH7.4,50%glycerol,0.09%sodiumazide.
BACKGROUND
PostsynapticDensityProtein95(PSD95),alsoknownasSynapseAssociatedProtein90kDa,isamemberofthemembrane-associatedguanylatekinasefamilyofproteins.PSD95isascaffoldingproteininvolvedintheassemblyandfunctionofthepostsynapticdensitycomplex.ThisfamilyofproteinsconsistsofN-terminalvariablesegmentsfollowedbythreeamino-terminalPDZdomains,anupstreamSH3domain,andaninactivecarboxy-terminalguanylatekinasedomain.ThefirstandsecondPDZdomainslocalizeNMDAreceptorsandK+channelstosynapses,andthethirdbindstoneuroligins,neuronalcelladhesionmolecules.PSD95alsobindstoneuronalnitricoxidesynthase.PSD95isimplicatedinexperience-dependentplasticityandplaysanindispensableroleinlearning.MutationsinPSD95areassociatedwithautism.
SPECIFICATIONSUMMARY
Antigen:RecombinantratPSD95
HostSpecies:Mouse
GeneID:29495
AccessionNo.:NP_062567.1,P31016
Specificity:Thisantibodyrecognizesmouse,rat,pig,andhumanPSD95.
APPLICATIONS
Immunoblotting:useat1ug/ml.Abandof
~100kDaisdetected.
DetectionofPSD95inratbrainmembranewith
#56457at1ug/ml.DetectionofPSD95inpiglethippocampuswith
#56457at1ug/ml.Lane1:mwMarkers,Lane2:
mousebrain,Lane3:pigletliver,Lanes4-15:hippocampusof12differentpiglets.Courtesyof
S.Fleming,U.ofIllinois.
Immunocytochemistry:useat1-10ug/ml.
Immunofluorescence:useat10ug/ml.
DetectionofPSD95inratneocortexwith#56457at1ug/ml.
DetectionofPSD95inmousebackskinwith#56457at10ug/ml.
DetectionofPSD95inhumanHaCaTcellswith#56457at10ug/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
