productdatasheet
CatalogNo.:56474(clone1D4)Size:100uginPBS,pH7.4;50%
glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
BACKGROUNDRhodopsiniscomprisedoftheproteinopsinandareversIBLycovalentlyboundcofactor,retinal.Opsin,madeupofsevenmembrane-embeddedalphahelices,bindsretinal,aphotoreactivechromophore,inacentralpocket.Inadditiontobeingthepigmentoftheretinathatisresponsiblefortheformationofphotoreceptorcells,rhodopsinconveysinformationstoredinthechromophoretothesurfaceofthemoleculeuponlightabsorption.Mutationsintherhodopsingeneleadtoretinitispigmentosa.
ThisantibodyrecognizestheC-terminalaaTETSQVAPA(COOH)ofnativeandrecombinantvertebraterhodopsin.
Immunoblotting:useat1-10ug/ml.Abandof~40kDaisdetected.
Immunohistochemistry/immunocyto-chemistry:useat1-10ug/mlImmunoprecipitation:useat1-2ugper100-500ugofprotein.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Rateyelysate
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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