ADVANCEDRESEARCHTECHNOLOGIES
CatalogNo.:56481(clone4D2)Size:100uginPBS,pH7.4;50%
glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
BACKGROUNDRhodopsiniscomprisedoftheproteinopsinandareversIBLycovalentlyboundcofactor,retinal.Opsin,madeupofsevenmembrane-embeddedalphahelices,bindsretinal,aphotoreactivechromophore,inacentralpocket.Inadditiontobeingthepigmentoftheretinathatisresponsiblefortheformationofphotoreceptorcells,rhodopsinconveysinformationstoredinthechromophoretothesurfaceofthemoleculeuponlightabsorption.Mutationsintherhodopsingeneleadtoretinitispigmentosa.
Antigen:BovinerhodopsinHostSpecies:MouseAntibodyClass:IgG1
ThisantibodyrecognizestheN-terminusofRhodopsinfromallmammalianspeciestested(human,mouse,rat,bovine,porcine),andmostfish,birds,andamphibians.
Immunoblotting:useat1-10ug/ml.Abandof~40kDaisdetected.
Immunohistochemistry:useat1-10ug/ml,paraffin-embeddedandfrozen.
Immunoprecipitation:useat1-2ugper100-500ugofprotein.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Rateyelysate
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
10919TechnologyPlace,SuiteCSanDiego,California92127Tel800-929-2114/858-675-2405Fax858-592-1509
Emailinfo@qedbio.comwww.qedbio.com
