productdatasheet
CatalogNo.:28113
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
AnovelTGF-betaup-regulatedmRNAspecies,theSin3-associatedprotein30-like,SAP30Lhasbeenidentified.ThepredictednuclearlocalizationsignalofSAP30LissufficientfornucleartransportoftheproteinalthoughmutatingitdoesnotcompletelyremoveSAP30Lfromthenuclei.ByreasonofitsnuclearlocalizationandclosehomologytoSAP30,itisthoughtthatSAP30LmighthavearoleinrecruitingtheSin3-histonedeacetylasecomplextospecificco-repressorcomplexesinresponsetoTGF-beta,leADIngtothesilencingofproliferation-drivinggenesinthedifferentiatingintestinalepithelialcells.
Lindfors,K.etal.BMCGenomics4(1),53(2003).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICATIONSUMMARYImmunogen:Syntheticpeptidecorrespondingtoaminoacids165-183(VKSNKSRLDQKSEGGKQLE)ofhuman
SAP30LconjugatedtoKLHattheN-terminus.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:None.Availableonrequest.
Thisantibodydetectswild-typetransfected,endogenous,orGST-SAP30Lfusionproteininhumancelllines.
Immunoblotting:useat1:500dilution.Bandsofapproximately21-22kDa(endogenous)and/or30kDa(wild-typetransfected)aredetected.
Positivecontrol:MCF7orHEK293celllysate.
DiluteinPBSormediumwhichisidenti-caltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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