productdatasheet
CatalogNo.:56469(cloneS22-21)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
Shankproteinsareafamilyofscaffoldproteinsidentifiedthroughtheirinteractionwithavarietyofmembraneandcyto-plasmicproteins.Shankproteinsatpostsynapticsitesofexcitatorysynapsesplayrolesinsignaltransmissionintothepostsynapticneuron.Shankproteinsarecrucialinreceptortyrosinekinasesignaling.RecentstudiessuggestthatdisruptionofglutamatereceptorsattheShank-postsynapticplatformcouldcontributetothedestructionofpost-synapticdensitywhichunderliessynapticdysfunctionandlossinAlzheimer’sdisease.Shank1mightberelevanttohumanautismspectrumdisordersduetoitsdifferentialroleinspecificcognitiveprocessesalongwithitsimportanceinsynapsestructureandfunction.
Thisantibodyrecognizeshuman,mouse,andratShank1.ItdoesnotreactwithShank2or3.
Immunoblotting:useat1-10ug/ml.Bandsof~190-220kDa(alternativesplicevariants)aredetected.
Adultratbrainmembraneprobedwith#56469
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Adultratbrain.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITYThisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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