ORDERINGINFORMATION

Catalogno.:56578(cloneS367-51)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

SHANKproteinsarescaffoldingadaptorsthathavebeenshowntointegrateneurotransmitterreceptorsintothecorticalCytoskeletonatpostsynapticdensities.SHANK1-3oftheSHANK/ProSAPfamilyaremolecularscaffoldsinthepostsynapticdensity(PSD).SHANKrecruitsbetaPIXandPAKtodendriticspinestoregulatepostsynapticstructureandinteractswithionotropicreceptorandmetabotropicglutamatereceptorcomplexes.TranscriptsplicevariationintheShankfamilyinfluencesthespectrumofShank-interactingproteinsinthePSDsofadultanddevelopingbraintoensurenormaldevelopment.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa538-626(SH3domain)ofratSHANK3.Thissequenceis100%identicalinmouse,97%identicalinhuman,and~70%identicalinSHANK1andSHANK2.

Accessionnos:NP_067708.1,Q9JLU4

GeneID:59312

HostSpecies:Mouse

AntibodyClass:IgG2a

Specificity:Thisantibodyrecognizeshuman,mouse,andratSHANK1/SHANK3.

APPLICATIONS

Immunofluorescence:useat10ug/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DetectionofSHANK1/SHANK3inneuroblastomacelllineSK-N-BEwith#56578at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)SHANK1/SHANK3stain.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.