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CatalogNo.:56466(cloneS23b-6)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

Shankproteinsareafamilyofscaffoldproteinsidentifiedthroughtheirinteractionwithavarietyofmembraneandcyto-plasmicproteins.Shankproteinsatpostsynapticsitesofexcitatorysynapsesplayrolesinsignaltransmissionintothepostsynapticneuron.Shankproteinsarecrucialinreceptortyrosinekinasesignaling.Shank2isexpressedinneuronsofthedevelopingretinaandcouldplayaroleinneuronaldifferentiationofthedevelopingretina.RecentstudiessuggestthatdisruptionofglutamatereceptorsattheShank-postsynapticplatformcouldcontributetothedestructionofpost-synapticdensitywhichunderliessynapticdysfunctionandlossinAlzheimer’sdisease.

Thisantibodyrecognizeshuman,mouse(weak),andratShank2.Itdoesnotcross-reactwithShank1orShank3.

Immunoblotting:useat1-10ug/ml.Abandof~160kDaisdetected.

Brainmembranesfromwholerat(RBM)andmouse(MBM)brainandfromhuman(HBM)cerebralcortexandhippocampus.

ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Adultratbrainlysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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