productdatasheet
CatalogNo.:56463(cloneS69-46)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
Shankproteinsareafamilyofscaffoldproteinsidentifiedthroughtheirinteractionwithavarietyofmembraneandcytoplasmicproteins.Shankproteinsatpostsynapticsitesofexcitatorysynapsesplayrolesinsignaltransmissionintothepostsynapticneuron.Shankproteinsarecrucialinreceptortyrosinekinasesignaling;specifically,Shank3canmediateErk-MAPKandP13Ksignalingwhichiscrucialfortubuleformation.
Shank3isalsooneofthelatestgenestobeassociatedwithautism.AmutationinasinglecopyofShank3onchromosome22q13canresultincommunicationdisorders.
(accessionno.Q9JLU4).HostSpecies:MouseAntibodyClass:IgG2b
SPECIFICITYThisantibodyrecognizeshuman,mouse,andratShank3.Itdoesnotcross-reactwithShank1orShank2.
Immunoblotting:useat1-10ug/ml.Abandof~40kDaisdetected.
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/ml
AdultrathippocampusImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:AdultratbrainorlysateofCOScellstransientlyexpressingShank3.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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