ORDERINGINFORMATION
CatalogNo.:2428
Format:100ug(1mg/ml)peptideaffinity-purifiedantibodyinPBS,0.02%sodiumazide.
BACKGROUND
Proteintyrosinephosphatases(PTPases),suchasSHP-1andSHP-2,arecriticalregulatorsintheintracellularsignalingpathwaysthatresultincellresponsessuchasmitosis,differentiation,migration,survival,transformation,ordeath.SHP-2isasignaltransducerforseveralreceptortyrosinekinasesandcytokinereceptors.AnovelSHP-2associatedglycoproteinwasclonedfromhuman,rat,mouse,andcattlebyseverallaboratoriesandwasdesignatedSIRPα,SHPS-1,andMyD-1.SIRPα/SHPS-1isasubstrateofmanyactivatedtyrosinekinasessuchasinsulinreceptor,EGFR,PDGFR,andsrc,andisaspecificdockingproteinforSHP-2.SIRPα/SHPS-1hasregulatoryeffectsoncellularresponsesandplaysageneralroleindifferentphysiologicalandpathologicalprocesses.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingto17aminoacidswithinthelast50aminoacidsattheC-terminusofhumanSIRPα.
Accessionno.:NP_542970
GeneID:140885
HostSpecies:Rabbit
Specificity:ThisantibodyrecognizeshumanSIRPalpha1,2,and3.
APPLICATIONS
Immunoblotting:useat0.5-2ug/ml.Abandof75-110kDaisdetected.
Immunocytochemistry:useat1-5ug/ml.
DetectionofSIRPαinTHP-1celllysatewith
#2428at0.5ug/ml.
DetectionofSIRPαinTHP-1cellswith#2428at1ug/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableat4˚Cforthreemonthsandforatleastoneyearat-20˚C.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
