ADVANCEDRESEARCHTECHNOLOGIES

CatalogNo.:56480(cloneSP-12)Size:100uginPBS,pH7.4;50%

glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

Synaptosomal-associatedprotein25(SNAP25)isamembraneboundproteinanchoredtothecytosolicfaceofmembranesviapalmitoylsidechainsinthemiddleofthemolecule.ItisacomponentoftheSNAREcomplexwhichisbelievedtoaccountforthespecificityofmembranefusionandforexecutingfusionbyformingatightcomplexthatbringsthesynapticvesicleandplasmamembranestogether.TwoalternativelysplicedisoformsofSNAP25havebeenidentified;SNAP25AandSNAP25B.Theseisoformsaredifferentiallyexpressedinneuronsandneuroendocrinecellsanddifferby9aminoacidsinthecentralportionoftheprotein.

Antigen:Crudesynapticpreparationfrompost-mortemhumanbrain.

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,rat,feline,andporcineSNAP25.

Immunoblotting:useat1-10ug/ml.Abandof~29kDaisdetected.

1. MWMarkers

2. Mouseliver

3. Mousebrain

4. Ratbrain

ELISA:useat1-5ug/mlImmunohistochemistry:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Ratbrainlysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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