CatalogNo.:56513(cloneS6-38)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

VAChTisamemberofthevesicularaminetransporter(VMAT)family.Theencodedtransmembraneproteintransportsacetylcholineintosecretoryvesiclesforreleaseintotheextracellularspace.AcetylcholinetransportutilizesaprotongrADIentestablishedbyavacuolarATPase.Thisgeneislocatedwithinthefirstintronofthecholineacetyltransferasegene.

Antigen:Syntheticpeptidecorrespondingtoaa521-532(CEDDYNYYYTRS)ofhumanVAChT(accessionno.NP_003046)

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,andratVAChT.

Immunoblotting:useat1-10ug/ml.Abandof55kDaisdetected.ThemolecularweightofglycosylatedVAChTis70kDa.

WesternblotanalysisofVAChTonratbrainmembranewith#56513at1ug/ml.

Immunohistochemistry:useat1-10ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:ratbrain

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.