CatalogNo.:56514(cloneS28-9)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

VGlut1isexpressedinasubsetofglutamateneuronswhereittransportsglutamateintonativesynapticvesiclesfromthebrain.GlutamatetransportbyVGlut1issaturatedwithaK(m)ofapproximately2mM.Whereasplasmamembraneglutamatetransportersrecognizebothaspartateandglutamateassubstrates,VGlut1recognizesonlyglutamate.

Antigen:Fusionproteincorrespondingtoaa493-560(cytoplasmicC-terminus)ofratVGlut1(accessionno.NP_446311).

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,andratVGlut1.Itdoesnotcross-reactwithVGlut2.

Immunoblotting:useat1-2ug/ml.Abandof~52kDaisdetected.

Adultratbrainimmunoblot

Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:ratbrainlysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.