VGlut2TransporterMonoclonalAntibody
CatalogNo.:56515(cloneS29-29)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.
PurifiedbyProteinGaffinitychromatography.
TheATP-dependent,chloride-sensitivevesicularglutamatetransportersincludeVGlut1,VGlut2,andVGlut3.VGlut1andVGlut2areexpressedinbraininacomplementaryfashion.VGlut1isexpressedinthetelencephalicregionswhereasVGlut2isexpressedinthelowerbrainstemanddiencephalicregions.
VGlut3isexpressedinasmallnumberofneuronsinthestriatum,hippocampus,cerebralcortex,andraphenuclei.
PancreaticαandβcellsexpressVGlut1andVGlut2inresponsetoglucoseconcentrations.
Antigen:Fusionproteincorrespondingtoaa501-582(cytoplasmicC-terminus)ofratVGlut2(accessionno.NP_445879).
HostSpecies:Mouse
AntibodyClass:IgG1
Thisantibodyrecognizeshuman,mouse,andratVGlut2.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof~60kDaisdetected.
Adultratbrainimmunoblot
Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:ratbrainlysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleast1yearat
-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
