VGlut3TransporterMonoclonalAntibody
CatalogNo.:56521(cloneS34-34)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.
PurifiedbyProteinGaffinitychromatography.
TheATP-dependent,chloride-sensitivevesicularglutamatetransportersincludeVGlut1,VGlut2,andVGlut3.VGlut3isamultipassmembraneproteinrestrictedtosynapticvesiclesofglutamatergicneurons.Itisspecificallyexpressedinamygdala,cerebellum,hippocampus,medulla,spinalcord,andthalamus.
Antigen:Fusionproteincorrespondingtoaa546-588(cytoplasmicC-terminus)ofratVGlut3(accessionno.NP_74947.1).
HostSpecies:Mouse
AntibodyClass:IgG1
Thisantibodyrecognizeshuman,mouse,andratVGlut3.Itdoesnotcross-reactwithVGlut1orVGlut2.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof~65kDaisdetected.
Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:CV-1fibroblaststransfectedwithVGlut3.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleast1yearat
-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
