ORDERINGINFORMATION

CatalogNo.:43055

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Akt,alsoknownasAkt1andProteinkinaseB(PKB),isaserine/threonine-specificproteinkinasethatplaysakeyroleinmultiplecellularprocessessuchasglucosemetabolism,apoptosis,cellproliferation,transcriptionandcellmigration.Akt1isalsoabletoinduceproteinsynthesispathwaysandis,therefore,akeysignalingproteininthecellularpathwaysthatleadtoskeletalmusclehypertrophy,andgeneraltissuegrowth.Sinceitcanblockapoptosis,andtherebypromotecellsurvival,Akt1hasbeenimplicatedasamajorfactorinmanytypesofcancer.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsitesofserinethreonine308(M-K-T(p)-F-C)derivedfromhumanAktandconjugatedtoKLH.

Accessionno.:P31749,NP_001014431.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratAktonlywhenphosphorylatedatthreonine308.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~60kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofAkt(phospho-Thr308)inextractsofHeLacellsuntreatedortreatedwithEGF,serumorboth.

DetectionofAkt(phospho-Thr308)inparaffin-embeddedhumanlungcarcinomatissue.

DetectionofAkt(phospho-Thr308)inmethanol-fixedHeLacellsshowingnucleardotstaining.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.