ORDERINGINFORMATION

CatalogNo.:43032

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

ATF2isatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteinthatcanperformmultiplefunctions.ItcanbindtothecAMP-responsiveelement(CRE),anditcanformahomodimeroraheterodimerwithc-JunandstimulateCRE-dependenttranscription.ATF2isalsoahistoneacetyltransferase(HAT)thatspecificallyacetylateshistonesH2BandH4invitro.ATF2mayalsobeinvolvedinacell'sDNAdamageresponseindependentofitsroleintranscriptionalregulation.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsitesofthreonine73or55(T-P-T(p)-R-F)derivedfromhumanATF2andconjugatedtoKLH.

Accessionno.:P15336,NP_001871.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,rat,andmonkeyATF2onlywhenphosphorylatedatthreonine73or55.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~65-75kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useat1:100-1:200.

DetectionofATF2(phosphor-Thr73or55)inextractsofHepG2cellsuntreatedortreatedwithEGF.

DetectionofATF2(phospho-Thr73or55)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofATF2(phospho-Thr73or55)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.