ORDERINGINFORMATION

CatalogNo.:43053

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

ATF4encodesatranscriptionfactorthatwasoriginallyidentifiedasawidelyexpressedmammalianDNA-bindingproteinthatcouldbindatax-responsiveenhancerelementintheLTRofHTLV-1.ATF4belongstoafamilyofDNA-bindingproteinsthatincludestheAP-1familyoftranscriptionfactors,cAMP-responseelementbindingproteins(CREBs)andCREB-likeproteins.Thesetranscriptionfactorssharealeucinezipperregionthatisinvolvedinprotein-proteininteractionslocatedC-terminaltoastretchofbasicaminoacidsthatfunctionsasaDNAbindingdomain.DiseasesassociatedwithATF4includeleukoencephalopathywithvanishingwhitematterandT-cellleukemia.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsitesofserine245(N-R-S(p)-L-P)derivedfromhumanATF4andconjugatedtoKLH.

Accessionno.:P18848,NP_001666.2

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanATF4onlywhenphosphorylatedatserine245.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~45kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofATF4(phospho-Ser245)inextractsofHeLacellsuntreatedortreatedwithUV.

DetectionofATF4(phospho-Ser245)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.