ORDERINGINFORMATION

CatalogNo.:43068

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

TheproteinencodedbytheBADgeneisamemberoftheBcl-2familywhosemembersareregulatorsofprogrammedcelldeath.BADproteinpositivelyregulatescellapoptosisbyformingheterodimerswithBcl-xLandBcl-2andreversingtheirdeathrepressoractivity.Proapoptoticactivityofthisproteinisregulatedthroughitsphosphorylation.ProteinkinasesAKTandMAPkinase,aswellasproteinphosphatasecalcineurin,werefoundtobeinvolvedintheregulationofthisprotein.Alternativesplicingofthisgeneresultsintwotranscriptvariantswhichencodethesameisoform.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine136(S-R-S(p)-A-P)derivedfrommouseBADandconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratBADonlywhenphosphorylatedatserine136.

Accessionno.:Q61337,NP_031548.1

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abanof~23kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofBAD(phospho-Ser136)inextractsofHT29cellsuntreatedortreatedwithUV.

DetectionofBAD(phospho-Ser136)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.