ORDERINGINFORMATION

CatalogNo.:43066

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

B-celllymphoma-extralarge(Bcl-xl,orBCL2-like1isoform1)isatransmembraneproteininthemitochondria.ItisamemberoftheBcl-2familyofproteinsandactsasapro-survivalproteinbypreventingthereleaseofmitochondrialcytochromec,whichwouldleadtocaspaseactivation.Relativeamountsofpro-andanti-survivalBcl-2familyproteinsdefinewhetheracellwillundergocelldeath:ifmoreBcl-xLispresent,thenporesarenon-permeabletopro-apoptoticmoleculesandthecellsurvives.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine62derivedfromhumanBcl-xLandconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratBcl-xLonlywhenphosphorylatedatserine62.

Accessionno.:NP_612815.1

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abanof~30kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofBcl-xL(phospho-Ser62)inextractsofHeLacellsuntreatedortreatedwithEGF.

DetectionofBcl-xL(phospho-Ser62)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofBcl-xL(phospho-Ser62)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.