ORDERINGINFORMATION

CatalogNo.:43003

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

c-Junincombinationwithc-FosformstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-Jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunction

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine73(L-A-S(p)-P-E)derivedfromhumanc-JunandconjugatedtoKLH.

Accessionno.:P05412,NP_002219.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatserine73.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

Detectionofc-Jun(phospho-Ser73)

inPC14cellextractsuntreatedortreatedwithEGF.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

Detectionofc-Jun(phospho-Ser73)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.