ORDERINGINFORMATION
CatalogNo.:43024
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
c-Junincombinationwithc-Fos,formstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunction
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine239(G-E-T(p)-P-P)derivedfromhumanc-JunandconjugatedtoKLH.
Accessionno.:P05412,NP_002219.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatthreonine239.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
Detectionofc-Jun(phospho-Thr239)inextractsofHeLacellsuntreatedortreatedwithUV.
Detectionofc-Jun(phospho-Thr239)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
