ORDERINGINFORMATION

CatalogNo.:43038

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Elk-1isamemberoftheEtsfamilyoftranscriptionfactorsandoftheternarycomplexfactor(TCF)subfamily.ProteinsoftheTCFsubfamilyformaternarycomplexbybindingtotheserumresponsefactorandtheserumreponseelementinthepromoterofthec-fosproto-oncogene.Theproteinencodedbythisgeneisanucleartargetfortheras-raf-MAPKsignalingcascade.

Alternativelysplicedtranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine417(L-S-T(p)-P-V)derivedfromhumanElk1andconjugatedtoKLH.

Accessionno.:P19419,NP_001244097.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratElk1onlywhenphosphorylatedatthreonine417.

APPLICATION

Immunohistochemistry:useatdilutionof1:50-1:100.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofElk1(phospho-Thr417)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofElk1(phospho-Thr417)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.