ORDERINGINFORMATION

CatalogNo.:43063

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Ezrin(alsoknownascytovillinorvillin-2)isencodedbytheEZRgene.Thecytoplasmicperipheralmembraneproteinencodedbythisgenefunctionsasaprotein-tyrosinekinasesubstrateinmicrovilli.AsamemberoftheERMproteinfamily,thisproteinservesasanintermediatebetweentheplasmamembraneandtheactinCytoskeleton.Itplaysakeyroleincellsurfacestructureadhesion,migration,andorganization.TheN-terminalFERMdomainstronglybindssodium-hydrogenexchangerregulatoryfactor(NHERF)proteins(involvinglong-rangeallostery).TheC-terminalERMdomainbindstoactin,phosphatidylinositol(4,5)bis-phosphate(PIP2)andmembraneproteinslikeCD44andICAM-2.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine353(Q-D-Y(p)-E-E)derivedfromhumanEzrinandconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:ThisantibodydetectsendogenoushumanEzrinonlywhenphosphorylatedattyrosine353.

Accessionno.:P15311,NP_001104547.1

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~81kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofEzrin(phospho-Tyr353)inextractsof293cellsuntreatedortreatedwithEGF.

DetectionofEzrin(phospho-Tyr353)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.