ORDERINGINFORMATION

CatalogNo.:43059

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

FocalAdhesionKinase,FAK(alsoknownasPTK2proteintyrosinekinase2,PTK2)isaproteinthat,inhumans,isencodedbythePTK2gene.FAKisafocaladhesion-associatedproteinkinaseinvolvedincellularadhesionandspreADIngprocesses.IthasbeenshownthatwhenFAKisblocked,breastcancercellsbecomelessmetastaticduetodecreasedmobility.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine861(H-I-Y(p)-Q-P)derivedfromhumanFAK.

HostSpecies:Rabbit

Accessionno.:Q05397,NP_005598.3

Specificity:ThisantibodydetectsendogenoushumanFAKonlywhenphosphorylatedattyrosine861.

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~125kDaisdetected.

DetectionofFAKinextractsofHT29cellsuntreatedortreatedwithEGF.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.