ORDERINGINFORMATION

CatalogNo.:43041

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

GATA1encodesaprotein,GATA1,whichbelongstotheGATAfamilyoftranscriptionfactors.Thisproteinplaysanimportantroleinerythroiddevelopmentbyregulatingtheswitchoffetalhemoglobintoadulthemoglobin.MutationsinthisgenehavebeenassociatedwithX-linkeddyserythropoieticanemiaandthrombocytopenia.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine142(R-L-S(p)-P-D)derivedfromhumanGATA1andconjugatedtoKLH.

Accessionno.:P15976,NP_002040.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratGATA1onlywhenphosphorylatedatserine142.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofGATA1(phospho-Ser142)in

extractsofHL60cellsuntreatedortreatedwithPMA.

DetectionofGATA1(phospho-Ser142)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.