ORDERINGINFORMATION

CatalogNo.:43042

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

GATA1encodesaprotein,GATA1,whichbelongstotheGATAfamilyoftranscriptionfactors.Thisproteinplaysanimportantroleinerythroiddevelopmentbyregulatingtheswitchoffetalhemoglobintoadulthemoglobin.MutationsinthisgenehavebeenassociatedwithX-linkeddyserythropoieticanemiaandthrombocytopenia.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine310(K-A-S(p)-G-K)derivedfromhumanGATA1andconjugatedtoKLH.

Accessionno.:P15976,NP_002040.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratGATA1onlywhenphosphorylatedatserine310.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.

Immunofluorescence:useatdilutionof1:100-1:200.

DetectionofGATA1(phospho-Ser310)inextractsofHT29cellsuntreatedortreatedwithAnisomycin.

DetectionofGATA1(phospho-Ser310)inmethanol-fixedHeLacells.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.