ORDERINGINFORMATION

CatalogNo.:43007

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

GSK3actsasamultifunctionaldownstreamswitchthatdeterminestheoutputofnumeroussignalingpathways.TherearetwomammalianGSK-3isoformsencodedbydistinctgenes,GSK3alphaandGSK3beta,whicharestructurallysimilar,butfunctionallynon-identical.GSK3αisinhibitedbyphosphorylationatS21byAktandotherkinases.DysregulatedGSK3hasbeenimplicatedinseveraldiseasesincludingtypeIIdiabetes,Alzheimersdisease,andcancer.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine21(T-S-S(p)-F-A)derivedfromhumanGSK3αandconjugatedtoKLH.

Accessionno.:P49840,NP_063937.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratGSK3αonlywhenphosphorylatedatserine21anddoesnotdetectGSK3βphosphorylatedatSer9.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~51kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofGSK3α(Phospho-Ser21)in293cellextractsuntreatedortreatedwithserum.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofGSK3α(phospho-Ser21)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.