ORDERINGINFORMATION

CatalogNo.:43002

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

GSK3βparticipatesintheWntsignalingpathwayandisimplicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN.GSK3βphosphorylatesJUNatsitesproximaltoitsDNA-bindingdomain,therebyreducingitsaffinityforDNA.ItalsophosphorylatesCTNNB1/beta-cateninandMUC1inbreastcancercellsanddecreasestheinteractionofMUC1withCTNNB1/beta-catenin.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine9(T-T-S(p)-F-A)derivedfromHumanGSK3βandconjugatedtoKLH.

Accessionno.:P49841,NP_001139628.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratGSK3betaonlywhenphosphorylatedatserine9.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~46kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofGSK3β(phosphor-Ser9)

in293cellextractsuntreatedortreatedwithserum.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofGSK3β(phospho-Ser9)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.