ORDERINGINFORMATION

CatalogNo.:43060

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

TheIntegrinβ3(ITGB3)geneencodesglycoproteinIIIa(GPIIIa),thebetasubunitoftheplateletmembraneadhesiveproteinreceptorcomplexGPIIb/IIIa.Thealphasubunit,GPIIb,isencodedbytheITGA2Bgene.TheGPIIb/IIIacomplexbelongstotheintegrinclassofcelladhesionmoleculereceptorsthatshareacommonheterodimericstructurewithalphaandbetasubunits.GlycoproteinIIIaisalsothebetasubunitof2otherintegrins,fibronectinreceptorandvitronectinreceptorwhichhavedistinctivealphasubunits.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteoftyrosine773(P-L-Y(p)-K-E)derivedfromhumanIntegrinβ3.

HostSpecies:Rabbit

Accessionno.:P05106,NP_000203.2

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratIntegrinβ3onlywhenphosphorylatedattyrosine773.

APPLICATIONS

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~110kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofIntegrinβ3(phospho-Tyr773)inextractsof293cellsalongwiththesameantibodypreincubatedwithblockingpeptide.

DetectionofIntegrinβ3(phospho-Tyr773)inparaffin-embeddedhumanbreastcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.